FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27697806
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG9500.015.248589540-41
TATGCCG37700.013.4182148-49
CGCTAGT11900.013.176831
CTCCGCT119700.010.6783011
CCCGTAT35950.010.1773761
CTCCGGT98500.09.8892111
GTCCGGT44300.09.8679751
GTCCGTT50350.09.437231
GTCGCAT25250.09.2210141
TCGCATT32550.09.195622
CTCCTAT91100.08.9712721
CTCCGAT91850.08.9497511
ATCGCGT17000.08.93938942-43
CGTATGC55050.08.92897446-47
CCCGTCT92850.08.8533611
CCCCGCT92150.08.8174861
CGCGTAT17050.08.77382644-45
CTTTATT261300.08.6922551
CCCGAAT59600.08.6900891
CCCGTTT66900.08.6651821
Produced by FastQC (version 0.11.5)