FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb317.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb317.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29750105
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG10100.015.28323940-41
CCCGTAT35500.013.7861861
CGCTAGT12350.011.9269671
CCCGCAT57350.011.3506931
TATGCCG37500.011.336246548-49
CTCCGCT110100.010.9617951
CTCCGAT99250.010.9153981
GTCGCAT29600.010.7551971
GTCCGTT49500.010.5589981
GTCCGGT49550.010.1647681
CCCGTCT98700.010.109681
CTCGTAT80650.09.8978341
GTCGGAT42150.09.8074681
CCCCGCT92100.09.492781
ATCGCGT16650.09.41231742-43
CCCGTTT72800.09.3986691
CGCGTAT16900.09.27301144-45
GTCCGCA56400.09.2672061
CTCGCGT11403.45608E-119.1696571
CTCGACT47300.09.14145851
Produced by FastQC (version 0.11.5)