FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb316.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb316.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27844048
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC294370.10572097850140182TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG64800.028.36289446-47
CGTATGC87050.021.27593244-45
TCGTATG79150.020.9997542-43
ACGGCTA94400.018.74055732-33
CTCGTAT107850.016.20409242-43
GTATGCC113100.016.14457144-45
CCCGTAT33150.016.0536351
ATGCCGT114500.015.92723346-47
CGGCTAC109700.015.88867732-33
TGCCGTC128550.014.81673248-49
GCCGTCT129450.014.0165648-49
TCTCGTA124100.013.70132340-41
AGTCACG150150.012.59006928-29
ATCTCGT148800.012.12919340-41
TCACGGC156350.011.71014230-31
GTCCGGT45750.011.6323071
CTCGCGT11950.011.5310531
CTCCGCT111750.011.5228691
GTCACGG157600.011.42229428-29
CCGTCTT165500.011.33658750-51
Produced by FastQC (version 0.11.5)