FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb315.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb315.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29987095
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT34300.015.0989921
GTCGCGA6701.8189894E-1213.4739331
CTCGCGT11450.013.2788391
CGTATTA16100.012.977893
CTCCGCT110650.011.5079771
TATGCCG39950.011.47148646-47
CGCTACT22900.011.4115031
CCCGTTT75950.011.19800951
CGCTAGT14300.010.9646181
GTCCGGT49100.010.9348431
CCCGCGT13750.010.7120991
CCCCGCT87550.010.5826421
GTCGGAT42000.010.5208121
GTCCGTT53750.010.5192341
CGCTATT18750.010.3895851
CTCCGGT103800.010.2991351
CCCGTCT101450.010.2098661
CTCGTAT92700.09.9947161
CTCCGAT99650.09.7267671
TCGTATC61900.09.6704882
Produced by FastQC (version 0.11.5)