FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb314.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb314.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22593907
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG32150.020.16342446-47
CCCGTAT31450.013.4462481
TCGTATG50500.011.9898242-43
CGTATGC58400.011.95352444-45
CTCCGGT85100.011.8927361
CTCCGCT95450.011.54898551
CGTATTA11700.011.3647473
GTCGCGA5052.17056E-611.290721
CTCCGAT80600.011.2597861
GTCCGTT45600.010.6283811
CCCGTCT78250.010.5656621
CGCTACT17900.010.3524551
TCGTATC46200.09.8721552
CTCGTAT76150.09.7962881
CTCGTTT83150.09.7715991
CCCCGCT74650.09.4839281
CCCGTTT58650.09.4787191
GTCCGGT35950.09.384071
GTCCGCT62350.09.0686431
CCCGTTA15750.09.0504981
Produced by FastQC (version 0.11.5)