FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb313.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb313.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27607573
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGT43700.013.5911571
CCCGTAT41150.012.5859071
CGTATTA12700.011.5916453
CTCCGGT108250.011.5439831
GTCGCAT25850.011.0285531
TATGCCG27300.010.78555546-47
CCCGTTT74600.09.9360471
GTCGGAT50450.09.8890821
CTCCTAT83700.09.87760451
CTCGCGT12300.09.6574561
CCCGAAT67900.09.586911
CGCTACT20100.09.4556591
CTCCGAT92000.09.4512911
CCCGTCT99700.09.4362161
CCCCGCT95950.09.4088481
GTCCGTT55100.09.1407561
CCCGCAT60050.09.0202731
CCCGCGT16950.08.9703241
CTCCGCT113550.08.9129271
CTCCGTT96800.08.6881191
Produced by FastQC (version 0.11.5)