FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb312.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb312.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29069410
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG35800.015.18904846-47
CTCGCGT10150.014.0444731
CCCGTAT42800.012.6564321
GTCCGTT53950.012.0664451
CTCCGCT118350.011.8040021
GTCCGAA43550.010.8018281
CTCCGGT110400.010.4589341
CGCTAGT14350.010.2650251
GTCCGCA65050.010.0074511
CCCGATT37850.09.917711
GTCCGGT45200.09.7767541
CCCGACT56900.09.6871471
CGCTACT23250.09.6056131
CTCCGTT99900.09.5605041
CTCCGAT99050.09.5466021
CTCCTAT98800.09.5226641
CGTATTA16650.09.4120263
CCCGTTT76400.09.3914761
CTCGTAT91450.09.3527431
TCCGCTA17800.09.3418582
Produced by FastQC (version 0.11.5)