FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb311.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb311.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32121346
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG40000.015.73126746-47
CTCCGGT122050.012.0685721
CCCGTAT47750.012.0404841
CGTATTA16450.011.8358573
CTCCGCT129150.011.5890571
CTCGTAT97750.011.4230771
CTCCGTT113200.010.8294211
CCCCGCT102850.010.8104441
CGCTAGT15050.010.7343171
CTCCTAT112950.010.7271891
CCCGTTT81650.010.7076361
CTCCGAT113050.010.5916091
TCGTATC62850.010.5072252
GTCCGGT47600.010.3814581
CCCGTAC44850.010.2764061
GTCCGTT62950.010.2653831
CCCGATT43450.010.0607411
CCCGTCT107850.09.6924711
CTCGCGT13300.09.645931
GTCCGGA67600.09.6295461
Produced by FastQC (version 0.11.5)