FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb310.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb310.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30017062
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG11600.014.74004640-41
TATGCCG42400.013.83492448-49
CGTATTA11750.012.5287883
GTCCGGT48250.012.4078491
GTCGCAT26200.010.5184081
GTCGGTT70650.010.0206841
GTCGGAT51200.09.4657161
CCCGTAT48850.09.43475151
GTCCGTT62300.09.3808111
TCCGTAT67900.09.26876336-37
GTCCGGA64300.09.1629221
CCGTATC69600.09.07649338-39
CTCCGCT127700.08.9298391
ATCGCGT20350.08.75105542-43
CGTATCG23350.08.74696238-39
GTTTATT193150.08.6098711
GCCGTTT62950.08.6046341
GTCCGCT86800.08.4299361
GTCCGCG37200.08.302211
GTCCGCA71850.08.26621151
Produced by FastQC (version 0.11.5)