FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb309.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb309.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24674739
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG35650.013.323400548-49
GTCCGGT42900.012.1832891
GTCCGTT50550.011.1854891
AATCGCG16050.010.35722540-41
CGTATTA11200.010.1758893
GTCCGCA60150.09.8742361
GTCCGTA29100.09.7968711
CTCCGCT103750.09.5716611
TCGTATC55200.09.4672672
GCCGTTA11555.456968E-129.4618271
CGCTATT15900.09.2638971
CTCCTAT72700.09.2153921
CCCGTCT87000.09.1752761
CGTATGC55550.09.14779146-47
GTCCGCT68050.09.0072191
TCCGCTA16050.08.8800872
GTCGGTT57300.08.8727511
CTCGTAT84500.08.8281981
ATCGCGT18100.08.7893742-43
GTCCGGA56900.08.7681141
Produced by FastQC (version 0.11.5)