FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb308.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb308.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27343565
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG36850.016.947646-47
GTCCGGT44600.014.382241
CCCGTAT37800.012.3186161
CGCTAGT12900.011.0499211
TCGTATG56700.010.55327242-43
GTCCGTT55600.010.5113371
CCCGTCT94100.09.6948091
CTCCGGT105050.09.6793321
TATACGC16700.09.6684267
CTCCGCT112900.09.6376061
TCGTATC59650.09.5574852
CGTATGC67450.09.50496944-45
GTCGTTT90600.09.3875541
GTCCGCA63100.09.3372721
GTCGCAT26050.09.1198971
GTCCGTA30400.09.0652971
CTCCGTT89750.08.894111
CTCGTAT91800.08.7472521
CCCCGCT86550.08.7288641
GTCGGTT72750.08.6212171
Produced by FastQC (version 0.11.5)