FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb307.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb307.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29033955
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG38250.013.40923246-47
GTCCGGT47050.013.2293911
CCCGTAT44650.012.344251
CGTATTA14400.011.8722573
GTCGCAA20050.011.6120771
GTCGCAT26700.010.8554231
GTCCGTT58500.010.7212711
GTCCGCA67150.010.6846211
GTCCGGA65500.010.3734441
TCCGCTA20300.010.2975332
GTCCGCT77750.010.0835091
GTCCGAA45850.010.0521881
GTCCGTA33800.09.8403261
CTCCGCT119500.09.7415121
GTCGCGA6857.860508E-79.7110381
GTCGGTT76700.09.5401141
CCCGTTT73700.09.4771561
CTCCGGT107650.09.3572881
GTCCAAT104850.09.1086871
GTCGTTT95200.09.0337871
Produced by FastQC (version 0.11.5)