FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb306.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb306.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22874832
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG26800.015.32836146-47
CGTATTA9900.013.9106913
CCCGTAT29150.012.87733651
GTCCGGT31400.012.2572461
CTCCGCT76850.011.3147451
CTCATAT100250.010.7117791
GTCCGTT43950.010.48697951
GACGTAT21500.010.38110116-17
GTCCGAA32150.010.3455741
CGATCTA12400.09.957234
GTACACT156400.09.9345581
CGCTAGT9357.858034E-109.6556051
GTCGTTT70600.09.6242941
CTCGTAT78500.09.4426191
ACGTATA24950.09.42195418-19
CTTTATT290900.09.4084111
CTCCGGT85050.09.274091
GTCGCGA4700.00165281439.0987591
CGTATAG24100.08.9660418-19
GTCCGGA45250.08.9256091
Produced by FastQC (version 0.11.5)