FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb305.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb305.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29284395
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG35950.015.25787546-47
GTACACT221450.014.2686831
CGTATTA14750.013.8440913
GTCGCAT23700.012.2298031
CCCGTAT39000.012.06171
CTCGCGT11200.011.8789481
GTCCGGT42050.011.8648211
ACTGTAG306350.011.424535
TGTAGCT368000.011.3688567
TCCGCTA17600.010.7974752
GTCCGTT61950.010.5846311
TACACTG297850.010.3997972
CCCGTCT96000.010.3940791
CCGTATT54050.010.3719692
GTCCGCT82900.010.2024261
CTCCGGT111400.010.1941411
TAGCTGT396500.010.1443579
CACTGTA359850.010.1087164
TAAGTAC142000.09.765233
CTGTAGC365450.09.4080446
Produced by FastQC (version 0.11.5)