FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb304.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb304.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27877644
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACT219950.015.3162961
GTCGCAT24050.013.2370491
GTCCGGT41150.012.4705431
ACTGTAG302650.011.9092815
TGTAGCT365900.011.7844117
TATGCCG34800.011.25872446-47
TAAGTAC142050.011.1991663
CGCTAGT11950.011.1332411
TACACTG299800.011.0611022
TCGTATG62700.010.90662442-43
CACTGTA350250.010.819554
TAGCTGT395500.010.4821579
GTCCGCA65300.010.3325291
CGTATTA12000.010.2890253
CCCGTAT36950.010.2874331
GTCCGGA61200.010.0930731
ACACTGT335900.010.0800263
CTGTAGC362000.010.0354616
GTCCGTT61000.09.9703771
GTAAGTA155550.09.92631152
Produced by FastQC (version 0.11.5)