FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb303.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb303.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32244698
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT44750.015.28986551
TATGCCG37650.014.505959546-47
CGTATTA14450.013.1455823
TAAGTAC131750.011.8225423
CTCCGCT126000.011.3131861
CTCCGGT126700.011.0256681
CGCTACT21300.010.9307681
CTCCGTT105800.010.6887151
GTCCGGT50400.010.2761431
CTCCGAT115750.010.0983011
CCCGTTT88750.010.0116911
GTACACT199500.09.9317871
GTAAGTA150300.09.9255452
CCGTATT64450.09.730492
CTCCCGT96350.09.6658161
CTCCTAT89700.09.587831
CTCATAT135250.09.5206411
CTCGCGT14600.09.4379851
CTCGTTT109700.09.269181
GTCCGTT69300.09.2562431
Produced by FastQC (version 0.11.5)