FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb286.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb286.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21943039
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG63650.022.05175448-49
AACGATC78250.019.60615238-39
ATCGCGT23850.017.62210542-43
CGTATGC84850.016.84747746-47
CGCGTAT25150.016.61665544-45
GAAACGA94000.016.34634236-37
AAACGAT87200.016.15036636-37
TGAAACG89900.015.58583434-35
ACGTGAA113300.015.42593532-33
CGTGAAA101800.015.37238532-33
AGTCACG148200.014.85500228-29
CGTATTA12700.014.58330253
GTCACGT133400.014.54478828-29
TCACGTG141450.014.40445430-31
CGATCGC32750.014.06739940-41
CACGTGA133200.013.99516330-31
TCGTATG71650.013.653687544-45
GTCCGGT38100.013.5939591
ATGCCGT102200.013.17608148-49
GTCCGTT43800.013.0182191
Produced by FastQC (version 0.11.5)