FastQCFastQC Report
Sat 1 Aug 2020
HKLHFDRXX_n01_mb285.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLHFDRXX_n01_mb285.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28753817
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA18150.017.5302453
CCCGTAT43350.013.9207321
GTCCGTT51350.013.0474621
GTCCGGT49350.012.5170971
GTCGCGA7301.09139364E-1011.7164771
CCGTATT46450.011.660022
TATGCCG37300.010.95152548-49
CTCCGCT119900.010.8983551
GTCCGCT66000.010.4393021
TTAACCG29350.010.3553127
CTCCGGT105200.010.2983231
TAACCGT27950.010.1943078
GTCCCGT65900.010.0946211
GTCCGGA62000.010.0398451
CCCGTCT96250.09.9229941
GTCCGAA47050.09.8972341
GTCGCAT29400.09.8589331
GTTCGTT46500.09.8099251
GTCGTAA37450.09.76981451
GTCCAAT109900.09.7281941
Produced by FastQC (version 0.11.5)