FastQCFastQC Report
Wed 19 Feb 2020
HKLG2DRXX_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLG2DRXX_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937533633
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAG172484151.8397649313984663No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCA127555781.3605461768004647No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG122812851.3099567383733528No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCTGGTGAGATGGCTC31958260.3408758776763799No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAACAAGCGAGATAGGAA30776190.3282675833347944No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCTCCGCCAGATCGCCGC29507280.31473302888964194No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTGGTGAGATGGCTCAGT28048850.2991770002986122No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCTCGGTCTTAGCGCCAT25813040.27532921584264913No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCTCCTCGTTGGGCG23244740.247934998615671No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCTTCGGACTTGGGCTTG21346500.22768783165350187No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGAGAGATGGCTCAG20862520.22252556351757036No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTCCTGTCCCGGCCGCCA18040320.1924231767800484No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGAGAGATGGCTCA17230680.18378732659290103No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTCAGGCAGCGAGAG16554740.17657755857810384No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC16040900.17109679520158613No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA13950380.14879871514966758No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGCGAGATGGCTCA10982480.1171422508316563No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCTCGAACCAGGCTGCTT10856430.11579776573199482No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTGGTGAGATGGCTCAGTG10759930.11476846932487593No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGTAGTGGTGGCGCACG9683260.1032844013181125No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCTTTGGCCTCGCCGGTA9592830.10231984925494401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT247715200.083.225951
AGCAGTG254193750.081.11822
GGTATCA254688550.080.657918
GTGGTAT254823200.080.651586
GTATCAA255663600.080.30119
TGGTATC256156700.080.184057
AGTGGTA257101450.079.986775
GCAGTGG261777300.078.8676453
CAGTGGT265189550.077.695414
ATCAACG254401950.041.19280610-11
CAACGCA255140950.041.10390512-13
ACGCAGA255403600.041.02744714-15
AGTACAT254186600.040.814520-21
TACATGG254453000.040.6863222-23
AGAGTAC256671300.040.58931718-19
GCAGAGT258210050.040.4829816-17
TCAACGC254358600.040.47720712-13
TATCAAC254463600.040.39896410-11
CATGGGG190662750.040.37664824-25
AACGCAG255833550.040.22611614-15
Produced by FastQC (version 0.11.5)