FastQCFastQC Report
Wed 19 Feb 2020
HKLG2DRXX_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLG2DRXX_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937533633
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGGTGA16345325917.43438883114714RNA PCR Primer, Index 38 (100% over 8bp)
AGCCAATT15805267116.858346776771047No Hit
GATACGCC13782772214.701096275230906No Hit
TTGAGATC13194495114.073623212619191No Hit
CGCTATGT12073050512.87745855193229No Hit
GCTGTCCA11714734812.495268849730962No Hit
AAACCGAG716425867.641601696010836No Hit
GGGGGGGG116258571.2400469264018392No Hit
TCGTTCAG23717240.2529748178111494No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)