FastQCFastQC Report
Wed 19 Feb 2020
HKLG2DRXX_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLG2DRXX_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937533633
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT2142250.017.6081641
CAATGGA2675050.017.589578
CTCGAAA2742350.017.534811
TGACGGC2239450.017.307081
GGAAAGC2440600.017.2921281
CGCTGGA2648400.017.2862971
ACTGAAC2499800.017.2747461
GACTAAC2624650.017.2220651
CCGGGAT2172000.017.1533051
GATGAAA2339650.017.0369931
ACGGAGA2844750.017.0319631
GACCTGG2790250.016.9853651
TTGAACG2216850.016.9628351
CTGCGGA1890450.016.9577221
GGCGACT2487000.016.8914831
ACGAGGA1745500.016.8639891
AGCTTGA2734250.016.8470691
GGGACCT2076150.016.8395461
TGAGCCG2644900.016.786911
TGCGTGG2169100.016.7796631
Produced by FastQC (version 0.11.5)