FastQCFastQC Report
Thu 20 Feb 2020
HKLCTDRXX_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLCTDRXX_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50791802
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC1209200.061.1095313
GCACACG1249750.060.17793711
CACACGT1242300.060.0550712
CACGTCT1220250.059.9692414
ATCGGAA1418950.059.862752
GATCGGA1432400.059.715251
TCGGAAG1404800.059.5551343
ACGTCTG1223500.059.17597215
CGGAAGA1402100.059.146444
CGTCTGA1222900.059.0307216
AGAGCAC1415200.055.3679288
AGCACAC1410400.054.48280710
GAACTCC1300650.053.02387621
CTGAACT1349900.052.32468419
TGAACTC1392450.050.40696320
GAGCACA1563200.049.7748769
GAAGAGC1625600.049.3887026
TCTGAAC1466150.048.64325318
AACTCCA1395450.048.48475322
GTCTGAA1503100.047.98402417
Produced by FastQC (version 0.11.5)