FastQCFastQC Report
Thu 20 Feb 2020
HKLCTDRXX_n01_GG101.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLCTDRXX_n01_GG101.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31673835
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG16000.017.49994733
CTCCGGT99000.013.2256691
CTCCGCT107500.012.4730171
CCCCGGT56050.011.8674991
CTCCGTT83800.011.8228891
CTCCGAT72600.011.7661511
CTCGCGA8050.011.7421891
TCGCGAA5301.0677468E-911.2289862
CCCCGTT51900.011.0626131
CTCCCGT82750.010.9998451
CTCGTAT62700.010.8321691
CCCGTAT37500.010.7361321
GACGTAT18000.010.49950417
CCCCCGT42850.010.4578011
CGCGCTA3752.911738E-510.2693431
CTCCGAA75150.010.2488451
CCCGAAT56050.010.24352551
CCCCGAT58750.010.13031
CGCTAGA14250.010.0728011
CTCCGGA104700.010.0647051
Produced by FastQC (version 0.11.5)