FastQCFastQC Report
Thu 20 Feb 2020
HKLCTDRXX_n01_GG100.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLCTDRXX_n01_GG100.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38711880
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG7200.020.9030541
TATGCCG27100.015.75652248
CTCCGGT127250.013.5630061
CTCCCGT101300.013.3396591
CTCCGAT90300.013.29761
CTCCGTT97350.013.0897791
CCCGGTT75200.012.1038181
CCCCGGT69750.011.7446071
CTCCGCT132050.011.5323471
CCCCGAT72500.011.4439821
CTCGCGA11650.011.4189071
CGCTAGT14850.011.0799621
ATCGCGT15700.010.92370942
CGTATTA14750.010.91498853
CTCGTAT77850.010.7924361
CTCCTAT89950.010.7806631
CTCGTTT105750.010.5272171
CCCCGCT114200.010.5146511
CCCCGTT63500.010.4196941
GTCCGTT59350.010.3814691
Produced by FastQC (version 0.11.5)