FastQCFastQC Report
Thu 20 Feb 2020
HKLCTDRXX_n01_GG099.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLCTDRXX_n01_GG099.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33893029
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG22700.015.264441548
CTCCGGT109200.013.6254021
CTCCGAT79750.013.4330241
CTCCCGT90950.013.2800431
CTCCGCT112100.012.6483091
CTCCGTT92450.012.3829431
CCCCGCT93700.012.3672031
CCCCGAT62500.011.8191291
CCCGTTT78050.011.7968451
CGCCTAT17300.011.7372121
CTCGTAT68550.011.3888651
CCCCGGT58500.011.1909971
CCCCCGT44850.010.9282011
GTCCGTT48750.010.8438971
CCCGTAT42050.010.7400621
CCCGAAT60550.010.5230191
CTCCTAT82350.010.415631
CTCCGGA109400.010.4003771
CCCGCAT54950.010.3849151
CGTATTA12850.010.3498823
Produced by FastQC (version 0.11.5)