FastQCFastQC Report
Thu 20 Feb 2020
HKLCTDRXX_n01_GG098.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLCTDRXX_n01_GG098.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38897673
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG24450.014.02874148
CTCGCGT12700.012.9555561
CTCCGGT123800.012.6966071
CTCCGTT103800.012.6135181
CTCCGAT87350.012.5041351
ACGTCCG28600.012.11524832
CCCGTAT47100.011.8921681
CTCCCGT96350.011.8811371
CTCCGCT131150.011.264351
CCCCGAT78750.010.7578811
CGTATTA14000.010.7496433
CTCGTAT82350.010.6701881
CCCCGCT112000.010.6272971
CTCCGGA131750.010.5753411
CCCCGTT63400.010.5464451
CCCCGGT71900.010.5168771
ATCGCGT13750.010.43655442
CCCCTAT50500.010.3289651
GTCCGTT55300.010.31868651
GTCCGGT51550.010.186491
Produced by FastQC (version 0.11.5)