FastQCFastQC Report
Thu 20 Feb 2020
HKLCTDRXX_n01_GG097.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLCTDRXX_n01_GG097.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16170157
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18050.036.4543148
CGTCCCG21500.031.41842535
ATCGCGT7900.025.69661342
ACCCGTC28700.023.7803532
CGCGTAT8100.023.33319944
CGTATGC29300.022.69625746
TCGTATG24850.019.57735345
GATCGGA38300.018.9208451
ACACGTC38850.018.3775913
ACGTCTG40350.017.78114515
GATCGCG12100.017.64490941
CCGATCG13050.017.43322839
ATCGGAA42300.016.8828332
GTATGCC40050.016.77903447
CGATCGC12950.016.48674840
CACCCGT43550.016.15375331
ATGCCGT41850.016.05735449
GTCCCGA44350.015.54708336
CGTCTGA48200.015.10308716
TCACCCG49100.014.82679930
Produced by FastQC (version 0.11.5)