FastQCFastQC Report
Thu 20 Feb 2020
HKLCTDRXX_n01_GG096.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLCTDRXX_n01_GG096.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12682301
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACA8650.013.3550451
CCCGTAT13650.012.8228591
CTCCGTT30800.012.2749741
CTCCCGT33350.011.7562741
AATCGCG4802.7139322E-911.66705241
CCCCGGT20150.011.4661171
GTCCGTT17550.011.3696011
CCCCCGT17450.011.2341471
TTCGCGA2500.001149758211.2018282
CCCCGCT31300.010.9604711
CTCCGGT39650.010.8594891
CTCGCGT3004.4373109E-410.5019221
CCCCGAT21700.010.485791
CGTCGAT7700.010.4564591
CGTATTA6402.0736479E-1010.3902323
CGCCTAT8450.010.3569251
CGCCAAT15250.010.3297591
CGACCGA5451.9694198E-810.277111
CGCATAT7503.6379788E-1210.2685451
CGCTAGA7603.6379788E-1210.1334321
Produced by FastQC (version 0.11.5)