FastQCFastQC Report
Thu 20 Feb 2020
HKLCTDRXX_n01_GG095.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLCTDRXX_n01_GG095.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10210707
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG2550.037.059341
TATGCCG8950.027.76531248
ATCGCGT4600.020.54374342
CGTATCG5200.018.1733139
CGCGTAT5200.018.17304244
CCGTATC16950.014.66095538
CGTATGC16850.014.33231846
TCCGTAT17150.013.8777337
AGTTCCG20550.013.62521334
CTCCCGT26600.013.5559251
GATCGGA20750.012.6536771
ACACGTC20850.012.25369513
GTTCCGT21000.011.99991935
CCCGTAT11000.011.7755891
CTCCGGT32400.011.6695021
ATCGGAA24100.011.4754812
CCCCGGT19700.011.37332251
CGCACGT7150.011.2614781
GTCCGAA12750.011.2576371
GTATGCC23050.011.23642347
Produced by FastQC (version 0.11.5)