FastQCFastQC Report
Thu 20 Feb 2020
HKLCTDRXX_n01_GG094.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLCTDRXX_n01_GG094.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12701791
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9950.015.82924348
CGTATTA7200.013.6108743
CGCCTAT6100.012.6258131
CCCGTAT14950.012.1766741
GTCCGGT15850.012.1478631
GTCCGTT17400.012.0717011
CTCCCGT33450.012.0356131
CCCCTAT19300.011.0646831
AATCGCG4752.8974682E-811.052917541
CCCCCCT31150.011.0137331
CCCCGCT34500.010.9590061
CTCCGAT33900.010.9464331
CTCCGCT42250.010.9373891
TAACGCG3853.280922E-610.90855816
CCCGAAT22050.010.7960981
CTCCGGT37250.010.6198571
CCCGCGT5956.4028427E-1010.5906361
CGCTATT7301.8189894E-1210.5503371
CGATTTA7700.010.4543634
TTAACCG10650.010.1874557
Produced by FastQC (version 0.11.5)