FastQCFastQC Report
Thu 20 Feb 2020
HKLCTDRXX_n01_GG091.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLCTDRXX_n01_GG091.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52573657
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC9082581.7275914437529047TruSeq Adapter, Index 11 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGTATGC840390.1598500176618872TruSeq Adapter, Index 11 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1012650.064.2257442
TCGTATG1013750.064.1508243
TCTCGTA1016200.063.99447641
ACGGCTA1135650.063.13920632
CGTATGC1129950.063.07058344
ATCTCGT1033750.062.93174440
CGGCTAC1130650.062.8828933
TATGCCG1135450.062.8424446
ATGCCGT1136900.062.75305647
TGCCGTC1147150.062.1526848
CATCTCG1047500.062.10233339
GCCGTCT1150200.061.9483149
CCGTCTT1151250.061.77932750
TCACGGC1167400.061.46996330
GTCACGG1170400.061.46638529
CGTCTGA1196100.061.4357416
AGTCACG1177650.061.1802828
CGTCTTC1163050.061.15848551
ACACGTC1212950.060.91123213
ACGTCTG1215000.060.5492115
Produced by FastQC (version 0.11.5)