FastQCFastQC Report
Thu 20 Feb 2020
HKLCTDRXX_n01_GG090.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLCTDRXX_n01_GG090.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67231920
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC37789585.620779534483026TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC3735030.5555441522419707TruSeq Adapter, Index 10 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4089250.066.46030442
TCGTATG4089050.066.4590643
TCTCGTA4092300.066.4099241
TATCTCG4096650.066.3180539
ATCTCGT4117000.066.030240
CGTATGC4547050.065.72153544
ATGCCGT4558450.065.6020547
TATGCCG4560200.065.5868646
CGTCTGA4651700.065.4325216
TGCCGTC4575250.065.3504648
CCGTCTT4558300.065.3170550
GCCGTCT4572700.065.25677549
ACACGTC4687000.065.22192413
ACGTCTG4676000.065.1209215
CGTCTTC4574200.065.0729751
CACGTCT4708100.064.8174214
GCTTATC4608300.064.73254436
GTATGCC4636550.064.5238645
TAGCTTA4656250.064.1145834
CACACGT4774450.064.1057412
Produced by FastQC (version 0.11.5)