FastQCFastQC Report
Thu 20 Feb 2020
HKLCTDRXX_n01_GG089.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLCTDRXX_n01_GG089.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59942893
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC664368711.08336062458647TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCGCGTATGC5739630.9575163481015172TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG895500.1493921889956162TruSeq Adapter, Index 9 (100% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG7294400.065.2773343
CTCGTAT7298750.065.2701642
TCTCGTA7301750.065.2538941
GATCTCG7320450.065.17422539
ATCTCGT7321800.065.09862540
CGGAAGA8380650.064.958014
ATCGGAA8441600.064.936542
TCGGAAG8418900.064.8919453
GATCGGA8481600.064.696371
ACACGTC8249400.064.6555313
CGTCTGA8203250.064.5388716
CACGTCT8260450.064.40507514
ACGTCTG8239300.064.38434615
CGTATGC8046350.064.3609144
TATGCCG8052300.064.34296446
ATGCCGT8052450.064.3273747
CGATCAG8088350.064.24734533
ACGATCA8117000.064.242732
CCGTCTT8024100.064.1721650
CACGATC8126100.064.1642931
Produced by FastQC (version 0.11.5)