FastQCFastQC Report
Thu 20 Feb 2020
HKLCTDRXX_n01_GG088.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLCTDRXX_n01_GG088.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70695296
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC19759572.7950332084329914TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATGC3056670.4323724735518471TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTGC2708080.38306367654221296TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATTC2121280.30005956831979314TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC1661360.23500290599250054TruSeq Adapter, Index 8 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG3242400.065.711639
CTCGTAT2430700.065.3763142
TCTCGTA2441200.065.3560641
TCGTATG2192900.065.3198243
ATCTCGT2837450.064.64667540
TATGCCG2352600.064.40797446
CGTATGC2364650.064.2584944
ATGCCGT2291700.064.1371847
CCGTCTT2533800.063.91417350
CGTCTTC2463200.063.7296651
TGCCGTC2582100.063.492648
GCCGTCT2330400.063.3394249
CGTCTGA3852750.062.57630516
GTATGCC2447750.062.079845
ACGTCTG3896500.061.9078215
TTGAATC3759100.061.87669436
GAATCTC3445500.061.8686138
ACACGTC3918600.061.75874713
CACGTCT3931900.061.4626114
TGAATCT3503800.060.8301837
Produced by FastQC (version 0.11.5)