FastQCFastQC Report
Thu 20 Feb 2020
HKLCTDRXX_n01_GG087.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLCTDRXX_n01_GG087.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63089624
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC20370043.228746457579142TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC1734000.27484709688553544TruSeq Adapter, Index 3 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2208350.065.93426542
TCGTATG2209150.065.8964643
TCTCGTA2210350.065.86986541
CGTATGC2424700.065.20920644
ATCTCGT2235600.065.1211940
CGTCTGA2454650.065.0436216
ACACGTC2471750.064.8413613
ATGCCGT2442300.064.80408547
ACGTCTG2467400.064.7684915
GCCGTCT2441700.064.6995949
TATGCCG2446250.064.69514546
CCGTCTT2438600.064.6584250
CATCTCG2257300.064.4998239
TGCCGTC2456400.064.4207148
CGTCTTC2460950.064.06830651
CACGTCT2503550.063.9241414
ATCGGAA2608250.063.3979262
TCGGAAG2613150.063.1032753
CACACGT2545150.063.05389812
GTATGCC2511350.063.0456845
Produced by FastQC (version 0.11.5)