FastQCFastQC Report
Thu 20 Feb 2020
HKLCTDRXX_n01_GG086.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLCTDRXX_n01_GG086.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74825726
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC23057673.081516375798345TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTATGC1816560.24277211824179293TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2555800.065.51630442
TCGTATG2520550.065.4809543
TCTCGTA2564200.065.4354541
CGATCTC2655700.064.9522438
GATCTCG2659800.064.89554639
CGTATGC2737950.064.8442644
ACGATCT2662850.064.79755437
ATGCCGT2716850.064.7324747
TATGCCG2748600.064.5348246
ATCTCGT2626500.064.5083340
GCCGTCT2684900.064.39081649
TGCCGTC2751050.064.34376548
CCGTCTT2710950.064.3221550
CACGATC2909050.064.0083736
CGTCTTC2708150.064.0060651
TCACGAT2918800.063.7615935
ATCACGA2923250.063.73636234
CATCACG2956200.063.07449333
GTATGCC2837200.062.6079745
GCTTGAA2756050.060.20411759
Produced by FastQC (version 0.11.5)