FastQCFastQC Report
Thu 20 Feb 2020
HKLCTDRXX_n01_GG085.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLCTDRXX_n01_GG085.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70408768
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC50901537.229430573192248TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATGC4313930.6126978389964159TruSeq Adapter, Index 12 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGT740450.10516445906282582TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG5582500.065.9233139
TCGTATG5553200.065.8660943
CTCGTAT5565650.065.8411642
TCTCGTA5573650.065.80443641
ATCGGAA6338400.065.587932
CGTCTGA6200800.065.5586816
ACACGTC6219500.065.5460513
TCGGAAG6340150.065.483023
GATCGGA6362600.065.437751
ACGTCTG6217500.065.4287515
ATCTCGT5615200.065.3972940
CACGTCT6255650.065.10750614
CGTATGC6098450.065.049144
ATGCCGT6089700.065.0072147
CCGTCTT6045050.064.940850
GCCGTCT6054400.064.9312749
TATGCCG6114150.064.9052446
TGCCGTC6108450.064.81110448
CGTCTTC6049350.064.7806251
CACACGT6307100.064.75442512
Produced by FastQC (version 0.11.5)