FastQCFastQC Report
Thu 20 Feb 2020
HKLCTDRXX_n01_GG084.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLCTDRXX_n01_GG084.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77924469
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC35201144.517341016465572TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGC3161650.40573263322461656TruSeq Adapter, Index 7 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT3816500.065.57799542
TCTCGTA3817650.065.5417341
TCGTATG3818050.065.5300343
ATCTCGT3850400.064.9869940
CATCTCG3856700.064.8663139
CGTATGC4208150.064.8043844
ATGCCGT4214300.064.73929647
TATGCCG4222450.064.621846
TGCCGTC4226700.064.52122548
GCCGTCT4220600.064.5033549
CCGTCTT4216550.064.4507550
CGTCTTC4231700.064.2000851
CGTCTGA4376900.063.67458716
GTATGCC4311700.063.31866545
ACGTCTG4408700.063.2661115
ACACGTC4451550.062.9236413
CACGTCT4463600.062.6142114
GATCATC4367800.062.4557636
GCTTGAA4378650.061.8056959
CACACGT4560750.061.51986712
Produced by FastQC (version 0.11.5)