FastQCFastQC Report
Thu 20 Feb 2020
HKLCTDRXX_n01_GG083.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLCTDRXX_n01_GG083.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77942849
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC58497637.505195248893198TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATGC4621350.5929152012393081TruSeq Adapter, Index 6 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA6937700.068.084831
ATCGGAA6940150.068.037722
TCGGAAG6967800.067.680213
CGGAAGA7000400.067.252534
ACACGTC6894000.067.0883413
CGTCTGA6885900.066.8465816
ACGTCTG6896800.066.7840715
CACGTCT6923750.066.6555514
CTCGTAT6301100.066.47443442
TCGTATG6302350.066.46825443
TCTCGTA6302400.066.4523941
GCACACG6978500.066.4409511
CACACGT6972450.066.43439512
TATCTCG6303200.066.3639539
ATCTCGT6341050.066.0429440
CGTATGC6864800.065.8614644
CGCCAAT6853950.065.8559333
ATGCCGT6884300.065.72243547
TATGCCG6885500.065.7166246
ACGCCAA6897600.065.7030332
Produced by FastQC (version 0.11.5)