FastQCFastQC Report
Thu 20 Feb 2020
HKLCTDRXX_n01_GG082.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLCTDRXX_n01_GG082.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55756019
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC14435582.5890621782017833TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC1211260.2172429132718389TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1783050.066.665041
ATCGGAA1789300.066.364112
TCGGAAG1821600.065.0163043
ACACGTC1780900.064.8385113
CTCGTAT1591100.064.690842
CGTCTGA1776650.064.6685816
TCGTATG1591850.064.6523943
TCTCGTA1595050.064.5240241
ACGTCTG1788200.064.3037315
CGGAAGA1840050.064.166584
CGTATGC1751750.063.8221644
CACGTCT1807350.063.76375214
GATCTCG1616800.063.6711639
ATCTCGT1618850.063.59918240
ATGCCGT1765100.063.41111847
TATGCCG1769150.063.28771646
TGCCGTC1776200.063.0049948
GCCGTCT1773350.062.9779649
CCGTCTT1769850.062.9403450
CACACGT1845900.062.72218712
Produced by FastQC (version 0.11.5)