FastQCFastQC Report
Thu 20 Feb 2020
HKLCTDRXX_n01_GG081.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLCTDRXX_n01_GG081.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81066627
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC28947653.5708467308008265TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTATGC2608430.32176372652090235TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGATGC2539500.31326084407089TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTGC1855530.22888950344511064TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATTC1685850.2079585721507816TruSeq Adapter, Index 4 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3195450.065.307243
CTCGTAT3387800.065.19598442
TCTCGTA3403500.065.12662541
GATCGGA4637150.065.113141
ATCGGAA4635850.065.102642
CGTCTGA4517800.064.7598316
AATCTCG3990550.064.7552639
TCGGAAG4649350.064.741153
ACACGTC4542200.064.700913
CGTATGC3489850.064.5649844
ACGTCTG4535650.064.5535715
ATGCCGT3399450.064.5281747
GCCGTCT3354750.064.3989249
TATGCCG3497600.064.297546
CGGAAGA4673450.064.2185754
CCGTCTT3521500.064.2029950
ATCTCGT3696000.064.1856740
CGTCTTC3450950.064.1321451
TGCCGTC3617800.064.07674448
CACGTCT4590650.063.9013914
Produced by FastQC (version 0.11.5)