FastQCFastQC Report
Thu 20 Feb 2020
HKLCTDRXX_n01_GG080.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKLCTDRXX_n01_GG080.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94256193
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC38068734.038857160292905TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC3588290.3806954095843867TruSeq Adapter, Index 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4137850.065.7220442
TCGTATG4138850.065.7026543
TCTCGTA4139650.065.6706241
TATCTCG4149300.065.47488439
ATCTCGT4177650.065.0724440
CGTATGC4592100.064.83143644
CGATGTA4577350.064.7579634
ATGCCGT4605000.064.7131747
TATGCCG4618550.064.5263846
ACCGATG4613300.064.4809532
CCGTCTT4604550.064.4793750
TGCCGTC4622150.064.4768548
GCCGTCT4617400.064.4560449
CCGATGT4604050.064.4181433
CACCGAT4622400.064.3971731
CGTCTTC4630250.064.1236151
GTCACCG4677500.063.85173829
TCACCGA4690600.063.67500330
GTATGCC4718150.063.18544845
GTATCTC4347000.062.52528438
Produced by FastQC (version 0.11.5)