FastQCFastQC Report
Tue 28 Jan 2025
HKL2MBGYW_n01_AA99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKL2MBGYW_n01_AA99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45768776
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTGG3353870.7327856003839823TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTTGG2589080.565686965279561TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTTTG1742790.380781430554315TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTTG1661420.3630029345770575TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGGGG1301310.28432265700092135TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTTTGG638070.1394116373136131TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTTTTG594740.12994448442317968TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGATGG575730.12579099777542666TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGGTG536150.11714318075711705TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTGTGG499160.10906125171448763TruSeq Adapter, Index 10 (97% over 42bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)