FastQCFastQC Report
Tue 5 Nov 2019
HKKNFBGXC_n02_Pilot_SS2_421W_500W.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKNFBGXC_n02_Pilot_SS2_421W_500W.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395101565
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18353050.464514738128157No Hit
GTCATATATAGCCTCGTTTAATTGTTCATCTCTGATTCGATGATGTCTCC5875980.14872074728430904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA845400.019.212561
TTAGTCC885150.018.1081458
TAGTCCA916750.017.9374739
GGACCAT1400050.017.443866
GTCATAT2374800.017.0620351
GTCCACA1035300.016.48184411
ATACCGG1263550.016.0932856
GTTATAC1345200.016.033743
GTATCAA3349400.015.80277251
ATTAGTC1052600.015.6133917
TCAGGGG1201150.015.53900418
TGGACCA1825000.014.827065
GGGGGTA1279100.014.79910521
AGTCCAC1188400.014.48690910
CCACAAT1129850.014.40984313
GGTATTC1272500.014.37343424
CAGGGGG1308400.014.24739119
CCTCGTT2202650.014.20195312
CAACGCA3561800.014.0662134
GTCACGA575750.013.85973833
Produced by FastQC (version 0.11.5)