FastQCFastQC Report
Tue 5 Nov 2019
HKKNFBGXC_n01_Pilot_SS2_421W_500W.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKNFBGXC_n01_Pilot_SS2_421W_500W.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395101565
Sequences flagged as poor quality0
Sequence length20
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCACAC5643150.013.920571
TCTCGAC8575650.013.9103692
CCACACT5674850.013.9010172
CTCCTAC5116800.013.8887294
TCTCCTA5126100.013.8737053
CCTACGT5116350.013.8557736
CACACTT5689450.013.8555223
AACTACC5257350.013.8515076
AATCCTT4622900.013.8511462
TCCTTAC4617900.013.8510194
TCAGAAC5263700.013.8503772
TCCACCT2844250.013.8493463
CTCTAGT5136400.013.8480721
GTCTCCT5138150.013.8471042
CGACGCT8613300.013.8422245
TAAGCGT5098400.013.8410011
TCAACCG3974050.013.8363061
GTCCTCT4545100.013.8350252
ACACCAT7662150.013.8345733
TCCTACG5129400.013.8338655
Produced by FastQC (version 0.11.5)