FastQCFastQC Report
Tue 5 Nov 2019
HKKNFBGXC_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKNFBGXC_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395101565
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18353050.464514738128157No Hit
GTCATATATAGCCTCGTTTAATTGTTCATCTCTGATTCGATGATGTCTCC5875980.14872074728430904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA844250.019.457541
TTAGTCC883950.018.0680058
TAGTCCA914900.017.9376839
GGACCAT1389800.017.3557916
GTCATAT2385450.017.2282981
GTCCACA1033800.016.44190811
ATACCGG1261450.016.134056
GTTATAC1343700.016.118743
GTATCAA3340800.015.8205021
TCAGGGG1198150.015.58705618
ATTAGTC1063650.015.3974327
GGGGGTA1282700.014.83810421
TGGACCA1821000.014.6555915
GGTATTC1270000.014.441524524
AGTCCAC1191200.014.40299210
CCACAAT1137600.014.34836513
CAGGGGG1305350.014.3109919
CCTCGTT2207250.014.29287812
CAACGCA3570450.014.0426154
TATACCG1634500.013.7652525
Produced by FastQC (version 0.11.5)