FastQCFastQC Report
Tue 5 Nov 2019
HKKNFBGXC_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKNFBGXC_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395101565
Sequences flagged as poor quality0
Sequence length8
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGCGA34097245886.29995125430597No Hit
GGGGGGGG4282368210.838651575576524No Hit
TAAGCGAA15910340.40268987545012636No Hit
TAAGGCGC15220490.3852298079355874No Hit
TAGGCGAA14128450.3575903325009609No Hit
AAGGCGAA5127420.1297747327323292No Hit
TTCGGCGC4817420.12192864890322568No Hit
TAAGGCAA3961830.10027371063437829No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)