FastQCFastQC Report
Tue 5 Nov 2019
HKKNFBGXC_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKNFBGXC_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395101565
Sequences flagged as poor quality0
Sequence length20
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCACAC5648100.013.9233011
TCTCGAC8592250.013.911412
CCACACT5683600.013.9020032
CTCCTAC5105050.013.8868324
TCTCCTA5111050.013.8776943
TCAGAAC5257800.013.8556382
TCCACCT2836900.013.8528833
AACTACC5257100.013.8524226
CACACTT5699500.013.8521933
AATCCTT4596850.013.8499612
CCTACGT5107650.013.8489396
TCCTTAC4592650.013.8459414
CGACGCT8629300.013.8431685
GTCTCCT5126000.013.842342
TAAGCGT5106550.013.8414641
CTCTAGT5115650.013.841071
GTCCTCT4560950.013.8409452
ACACCAT7669550.013.834713
TCAACCG3985150.013.8340671
TCCTACG5118100.013.8326835
Produced by FastQC (version 0.11.5)