FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78832256
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8429831.0693376579252025No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT79000.019.5667254
TCATTAA1106050.019.37880794-95
TATCATT1588350.018.22193594-95
GTATCAT1894500.017.28223894-95
CGTATCA2237200.015.93349294-95
ATCATTA1683900.015.66440894-95
AGTACCG89800.014.8376215
CCGTATC2585250.014.64016194-95
GTACCGT65400.014.1412516
CACTGAG140900.013.2862347
GCCGTAT3104100.013.225409594-95
TACCGTG79800.013.0657877
GTATAGG143200.012.843491
GGACCGT83150.012.71960456
GTCTATG125350.012.652581
GTCCTGG87150.012.6074091
CATTAAA1247500.012.59431294-95
ACTGTCC76500.012.5142438
CGCCGTA3655300.012.44244994-95
TAGACGG56000.012.3622945
Produced by FastQC (version 0.11.5)