FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n02_POK60-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n02_POK60-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91545817
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG84250.024.016345
ATCATTA3742000.023.6546594-95
TCATTAA2836600.023.27510894-95
TATCATT4808950.021.8910694-95
CATTAAA1979450.021.00391694-95
GTACCGT131900.020.0575356
GTATCAT5902500.020.02042894-95
TATACCG225150.019.1338545
GTCTAGG225500.018.864461
AGTACCG191850.018.1471885
ACTGTCC123100.017.6327518
CGTATCA7237100.017.4482794-95
CCTAGAC227500.017.4330043
GTATAAG390950.016.8759421
GTGTATG289700.016.5255931
TACCGTG170050.016.2557987
AAGACCG197300.016.1292765
GGACTGC164200.016.0830656
CTAGACA238150.015.95535854
TAGAGCG212400.015.7876445
Produced by FastQC (version 0.11.5)